Biotechnology http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/144 2026-04-19T15:37:57Z 2026-04-19T15:37:57Z Dhanushka Udayanga http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1596 2026-02-14T04:59:51Z 2014-01-01T00:00:00Z

Molecular phylogeny and taxonomy of plant pathogenic genera diaporthe (Phomopsis) and colletotrichum Dhanushka Udayanga Hyde,Kevin David The genus Diaporthe (Phomopsis) is an important genus of phytopathogenic fungi with a worldwide distribution. Cryptic diversification, phenotypic plasticity and extensive host associations have complicated accurate species identification in the genus. In this study, the phylogenetic species recognition of Diaporthe is re-evaluated with worldwide collections with special reference to the significant phytopathogens on crops, ornamentals and forest trees and species complexes. The Genealogical Concordance Phylogenetic Species Recognition (GCPSR) was applied to resolve the species limits with four gene phylogenetic analysis of ITS (nuclear ribosomal internal transcribed spacer), EF1-α (translation elongation factor 1-α), TUB (β tubulin) and CAL (calmodulin). The four gene combined phylogeny was used to define novel taxa from Thailand including D. ptercarpicola, D. siamensis and D. thunbergii. The species of Diaporthe associated with melanose and stem end rot of Citrus were re-defined, implementing the combined analysis of ITS, EF1-α, TUB, CAL and ACT (actin) genes and Genealogical Sorting Index methods. (6) Diaporthe citri, D. cytosporella, D. foeniculina and D. rudis, are epitypified with clarification of nomenclature and taxonomy. The Diaporthe sojae species complex associated with soybean, cucurbits, and other herbaceous hosts were resolved based on five gene analysis including ITS, EF1- α, TUB, CAL and HIS (histone-3) genes. The seed decay pathogen D. longicolla was distinguished from the pod and stem blight pathogen Diaporthe sojae. The Lima bean pathogen D. phaseolorum, other herbaceous crop pathogens including D. arctii, D. batatas and D. cucurbitae were resolved with epitypes designated with the introduction of a new species D. ueckerae from cucurbits in USA. The generic type species Diaporthe eres was redefined with the strict application of genealogical concordance and non-discordance criteria in conjunction with morphology with the epitypification of six phylogenetic species within the complex. Eight molecular markers including ACT, Apn2 (DNA-lyase), CAL, EF1-α, Fg1093 (ribosomal protein L37), HIS, ITS and TUB were used in the phylogenetic analyses and testing the informativeness. The caution is warranted using the ITS sequence data within the cryptic species complexes, and EF1-α is proved to be the best single marker to resolve the species. The utility of EF1-α, Apn2, HIS and ACT genes are found to be superior to the other genes used with reference to the phylogenetic informativeness. New primers were designed and made available for the amplification of Apn2, ACT and CAL genes within Diaporthe. Guidelines for the identification and description of new species are provided with the discussions on insights in to the evolution and the pattern of speciation within the genus. A molecular phylogenetic analysis of anthracnose pathogens of tropical fruits was performed, with fresh collection of economic and wild fruits in northern Thailand. The species within Colletotrichum gloesporioides species complex were found to be the dominant among anthracnose pathogens. A six gene combined phylogeny of ITS, ACT, TUB, CAL, GPDH (glyceraldehyde-3-phosphate (7) dehydrogenase), GS (glutamine synthetase) was used to resolve the C. gloesporioides species complex with the description of the new species, C. syzygicola. In general, this thesis provides a comprehensive account in terms of utility of molecular data to resolve plant pathogenic species complexes in fungi. A number of economically important species of common plant pathogenic genera, Diaporthe and Colletotrichum were re-defined with accurate phylogenetic placement, morphology and insights in to the speciation and evolution. Dissertation (Ph.D.) -- Biotechnology, School of Science. Mae Fah Luang University, 2014

2014-01-01T00:00:00Z Dimuthu Sandarenu Manamgoda http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1594 2026-02-14T04:08:44Z 2014-01-01T00:00:00Z

Molecular phylogeny and taxonomy of plant pathogenic complex of bipolaris, cochliobolus, curvularia and endophytic colletotrichum Dimuthu Sandarenu Manamgoda Hyde,Kevin David The plant pathogenic generic complex Bipolaris - Cochliobolus - Curvularia comprises many destructive plant pathogens that cause severe cereal crop losses worldwide. The taxonomy of this complex is confused as frequent nomenclatural changes occurred over the past 50 years. The Bipolaris - Cochliobolus - Curvularia complex is investigated based on a set of ex-type cultures and collections from northern Thailand and elsewhere. Combined gene analysis of ITS (nuclear ribosomal internal transcribed spacer), GPDH (glyceraldehyde 3-phosphate dehydrogenase), LSU (large subunit) and TEF (translation elongation factor 1-α) revealed the segregation of this generic complex into major two groups. Bipolaris and Cochliobolus species clustered in Group 1 along with their type species, whereas Curvularia species (including species named as Bipolaris, Cochliobolus and Curvularia) clustered in Group 2, with its generic type of Curvularia. The initial generic resolution was fundamental to the comprehensive assessments of taxonomy and phylogeny. The nomenclatural conflict in this complex is resolved giving priority to the more commonly used established generic names (6) Bipolaris and Curvularia, therefore the Cochliobolus names were recognised as synonyms. The genus Bipolaris is revisited with all available type materials and most of the additional materials selected from worldwide herbaria. A phylogenetic analysis of the genus provided with fresh collections and including the additional authenticated cultures. The generic type Bipolaris maydis is neo-typified with other ex, neo-types of B. chloridis, B. cynodontis, B.maydis, B. salviniae, B. victoriae, and B. yamadae. Lectotypes were designated for B. hadrotrichoides and B. stenospila. Modern descriptions and illustrations are provided for 46 species of Bipolaris, with a revision of synonyms, and notes on nomenclature, biology and ecology of the species. A taxonomic key is provided for the morphological identification of the species within the genus. The generic wide phylogenetic tree is provided for future studies within the genus. The genus Curvularia is revised in two consecutive phylogenetic assessments resulting 3 new species including C. asianensis, C. alcornii and C. boedjinii. Additionally the generic type C. lunats and C. kusanoi is neotypified, and the generic concepts were reassessed. Nineteen species previously thought to be Bipolaris are transferred to the genus Curvularia, broadening the species numbers within the genus based on phylogenetic and morphological deference. The endophytic Colletotrichum from several grass species were studied with ITS, ACT (actin), CAL (calmodulin) and GPDH phylogeny and morphology. A new species, C. endophytica is described with full descriptions and illustrations. This study revealed an unknown diversity of Colletotrichum species associated with tropical grass with perspectives for future studies. (7) In overall, this thesis serves as a complete account of graminicolous species complex Bipolaris-Curvularia, as well as endophytic Colletotrichum providing grounds for further study in plant pathology and fungal systematics. Dissertation (Ph.D.) -- Biotechnology, School of Science. Mae Fah Luang University, 2014

2014-01-01T00:00:00Z Kritsana Jatuwong http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1470 2026-01-06T09:31:01Z 2015-01-01T00:00:00Z

Optimizing gorwing conditions for the tropical mushrooms, Clitopilus species and Agaricus subrufescens Kritsana Jatuwong Pattana Kakumyan Thesis (M.Sc.) -- Bioscience, School of Science. Mae Fah Luang University, 2015

2015-01-01T00:00:00Z อังค์วรา ศรีเหรา http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1465 2026-01-06T09:08:06Z 2558-01-01T00:00:00Z

การวิเคราะห์ความแปรปรวนแบบเบสเดียวของยีน Waxy และ SSIIa ที่สัมพันธ์กับคุณสมบัติการย่อยของแป้งในข้าวไทย อังค์วรา ศรีเหรา สยาม ภพลือชัย วิทยานิพนธ์ (วท.ม.) -- สาขาวิชาเทคโนโลยีชีวภาพ, สำนักวิชาวิทยาศาสตร์. มหาวิทยาลัยแม่ฟ้าหลวง, 2558

2558-01-01T00:00:00Z