http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/276 2026-04-19T15:43:39Z http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1244 Cordyceps militaris extract Induces proliferation, migration and expression of growth factors in human dermal fibroblast cells for enhanced wound healing Kanjana Soodpakdee Sunita Chamyuang Thesis (M.Sc.) -- Biological Science, School of Science. Mae Fah Luang University, 2024 2024-01-01T00:00:00Z http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1242 Optimization on adenosine production from Cordyceps militaris using submerged fermentation Noppasorn Chuenprasert Sunita Chamyuang Thesis (M.Sc.) -- Biological Science, School of Science. Mae Fah Luang University, 2024 2024-01-01T00:00:00Z http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1104 Pectin from coffee Pulp: extraction, characterization, and bioactivities Kunakorn Deesophon Sunita Chamyuang Coffee pulp, a significant by-product of coffee processing in Thailand, represents a promising source of value-added compounds. This study investigates the physicochemical characteristics and bioactive properties, specifically the prebiotic and anticancer potential, of pectin derived from coffee pulp (CP). Pectin was isolated using a double extraction method and subsequently modified enzymatically (SFD). Comprehensive characterization involved thermogravimetric analysis (TGA) for purity, Fourier-transform infrared spectroscopy (FTIR) for the degree of esterification (%DE), gel permeation chromatography (GPC) for molecular weight, and high-performance anion-exchange chromatography (HPAEC-PAD) for sugar composition and structure. Bioactivity assessments included the DPPH assay for antioxidant potential, in vitro growth studies with Pediococcus sp. (PE), Lactococcus lactis (LL), and Lactobacillus acidophilus (LA) to determine Prebiotic Index and Activity Scores, and the CCK-8 assay for cytotoxicity against SW480 colon cancer and CCD 841 CoN normal colon cells, with apoptosis confirmed via Annexin V-FITC/PI staining. Results were benchmarked against commercial prebiotics (inulin), pectins (HMP, L20, L40), and 5- Fluorouracil (5-FU). The extraction yielded 7.99% low methoxy pectin (LMP) with 4.76% DE, 68.05% purity, a weight-average molecular weight (Mw) of 68.6 KDa, and a linear structure (CP). Enzymatic modification yielded SFD, which retained LMP characteristics (0.00% DE) but exhibited increased branching and a significantly reduced Mw of 1.10 KDa. SF demonstrated the highest antioxidant potential (IC50 of 101.33 µg/mL), surpassing commercial pectins which failed to reach 50% inhibition. Furthermore, CP significantly promoted the growth of all tested lactic acid bacteria, showing higher prebiotic index (PE: 0.95, LL: 3.88, LA: 3.73) and activity scores (PE: 0.25, LL: 0.40, LA: 0.72) than commercial pectins. Notably, only SFD exhibited significant anticancer activity, selectively inhibiting SW480 cell proliferation (IC50 of 3.40 mg/mL) more effectively than normal CCD 841 CoN cells (IC50 of 11.13 mg/mL), while unmodified CP showed no effect. SFD induced late-stage apoptosis in 19.80 ± 7.51% of SW480 cells, compared to 10.68 ± 1.82% in controls and 25.97 ± 3.51% with 5-FU. These findings highlight that coffee pulp pectin is a promising natural prebiotic for food applications and gut health support. Moreover, its enzymatically modified form (MCPP) shows potential as a selective anticancer agent, warranting further investigation for therapeutic applications. Thesis (M.Sc.) -- Biological Science, School of Science. Mae Fah Luang University, 2025 2025-01-01T00:00:00Z http://mfuir.mfu.ac.th:80/xmlui/handle/123456789/1082 Optimization and bioactivities of beta glucan extracted from Pleurotus ostreatus cultivated on germinated riceberry rice (Oryza sativa) Jutamat Nacha Sunita Chamyuang Beta-glucan (β-glucan), a polysaccharide recognized for its prebiotic effects and potential to inhibit colon cancer cell proliferation, is derived from sources like grains (β-1,3/1,4-glucan) and fungi (β-1,3/1,6-glucan). This study focused on optimizing the co-culture of germinated Riceberry rice with Pleurotus ostreatus mycelium to enhance the yield of this valuable bioactive compound. Optimal conditions for beta-glucan production were achieved after 9 days of co-cultivation, resulting in a substantial yield of 222.94 ± 2.33 mg/g of dried Riceberry rice. The extracted β-glucan was confirmed as a β-1,3/1,6-glucan using FTIR spectroscopy. Biological activity assessment demonstrated the extract's promising prebiotic properties, significantly promoting the growth of Lacticaseibacillus rhamnosus and Bacillus coagulans, as indicated by their respective Prebiotic Indices (6.36±0.72 and 115.70±10.19) and Prebiotic Activity Scores (0.56±0.03 and 1.39±0.06). Notably, the extract exhibited potent anti-colorectal cancer activity in vitro, inhibiting 66.23% of SW480 cell growth at a concentration of 1 mg/mL, a more pronounced effect than observed with standard beta-glucan and 5-fluorouracil. Additionally, the extract induced a significant level of apoptosis (28.91%) in these cancer cells. The formulated beta-glucan extract shows strong nutritional value, reinforcing its role as a promising health-promoting and anticancer nutraceutical. Thesis (M.Sc.) -- Biological Science, School of Science. Mae Fah Luang University, 2025 2025-01-01T00:00:00Z