Chemical constituents from the root of mucuna macrocarpa wall., antibacterial and antioxidation properties

Abstract

Chromatographic separation of the roots of Mucuna macrocarpa led to the isolation of nine compounds. They were four triterpenoids: lupenone, lupane, betulinic acid and lupeol, two steroids: stigmasterol and  -sitosterol, one anthraquinone: vismiaquinone C, one alkaloid: murrayanine and one benzaldehyde: 4-methoxybenzaldehyde. Their structures were elucidated on the basis of spectroscopic techniques. Bioactivity assays showed that the compounds lupenone and vismiaquinone C exhibited antibacterial activities against Bacillus cereus, Pseudomonas fluorescens and Salmonella typhimurium with the minimum inhibition concentrations (MICs) values of 32-64 μg/mL. The crude methanolic extracts (on 2002 and 2006) showed strong antioxidation activities (IC50 0.31 and 0.88 mg/mL, respectively). The crude acetone extracts (on 2002 and 2006) showed good antioxidation activities with the IC50 of 1.32 and 3.30 mg/mL, respectively. The crude dichloromethane extracts showed weak activity in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay. All of the compounds showed weak antioxidative activities when tested with DPPH radical and less than 10% for radical scavenging activity.